| Publication Type | J |
| Authors | Moghaddam, MSH; Safaie, N; Soltani, J; Pasdaran, A |
| Author Full Name | Moghaddam, Mandieh S. Hosseyni; Safaie, Naser; Soltani, Jalal; Pasdaran, Ardalan |
| Title | Endophytic association of bioactive and halotolerant Humicola fuscoatra with halophytic plants, and its capability of producing anthraquinone and anthranol derivatives |
| Source | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY |
| Language | English |
| Document Type | Article |
| Author Keywords | Humicola fuscoatra; Antibacterial; Antifungal; Antiproliferative; Anthraquinone; Anthranol; Aloesaponarin II; Chrysarobin |
| Keywords Plus | FUNGAL ENDOPHYTES; HYPERSALINE WATERS; DIVERSITY; ANTIFUNGAL; METABOLITES; COMMUNITY; IDENTIFICATION; CONSTITUENTS; PATHOGENS; EXTRACTS |
| Abstract | Halophytic plants growing in harsh desert environments are rich reservoirs of unique endophytic microorganisms. Here, healthy fresh plants of the families Tamaricaceae and Amarantaceae at three saline locations in Iran were investigated for their bioactive endophytic fungi. Among a vast number of isolates, eight isolates were identified as Humicola fuscoatra (Sordariomycetes, Pezizomycotina, Ascomycota) by microscopy and representative DNA sequences of the 5.8S rDNA (ITS) and partial beta-tubulin (TUB2). Those isolates were halotolerant, and highly bioactive, so that their intra- and extra-cellular metabolites possessed in vitro antifungal, antibacterial and antiproliferative activities, against a number of fungal and bacterial plant pathogens including the fungi Arthrobotrys conoides, Pyrenophora graminea, Pyricularia grisea and the bacteria Agrobacterium tumefaciens, Pseudomonas syringae and Xanthomonas oryzae. Chemical analyses of metabolites from the endophytes using HNMR, CNMR, NOESY, COSY, HMBC, HSQC, DEPT, TOCSY and EI MASS techniques identified 3,8-dihydroxy-1-methyl-9,10-anthracenedione (aloesaponarin II; an anthraquinone derivative), 1,8,9-anthracenetriol structure (chrysarobin; an anthranol derivative) and 2,4-di-tert-butylthiophenol in fungal extracts. To the best of our knowledge, this is the first report of endophytic association of halotolerant H. fuscoatra isolates with Tamaricaceae and Amarantaceae, and their bioactivity against plant pathogens. Also, the capability of chrysarobin and aloesaponarin II production is new to the fungal kingdom. These findings may find application in agriculture, pharmacology, and biotechnology. |
| Author Address | [Moghaddam, Mandieh S. Hosseyni; Safaie, Naser] Tarbiat Modares Univ, Fac Agr, Dept Plant Pathol, Tehran, Iran; [Soltani, Jalal] Bu Ali Sina Univ, Fac Agr, Dept Plant Protect, Sect Plant Pathol, Hamadan, Iran; [Pasdaran, Ardalan] Shiraz Univ Med Sci, Med Plants Proc Res Ctr, Shiraz, Iran |
| Reprint Address | Safaie, N (corresponding author), Tarbiat Modares Univ, Fac Agr, Dept Plant Pathol, Tehran, Iran. |
| E-mail Address | m.moghadam18@yahoo.com; nsafaie@modares.ac.ir; Soltani@basu.ac.ir; ardalan.pasdar@gmail.com |
| ResearcherID Number | Safaie, Naser/E-1269-2018; Pasdaran, Ardalan/O-2976-2017 |
| ORCID Number | Safaie, Naser/0000-0001-6065-7010; Pasdaran, Ardalan/0000-0001-6713-0756; Soltani, Jalal/0000-0001-9559-0071 |
| Funding Agency and Grant Number | Tarbiat Modares University |
| Funding Text | This research was funded by a Grant from Tarbiat Modares University to NS. |
| Times Cited | 1 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 2 |
| Publisher | SPRINGER |
| Publisher City | DORDRECHT |
| Publisher Address | VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS |
| ISSN | 0003-6072 |
| ISBN | 0003-6072 |
| 29-Character Source Abbreviation | ANTON LEEUW INT J G |
| ISO Source Abbreviation | Antonie Van Leeuwenhoek |
| Publication Date | FEB |
| Year Published | 2020 |
| Volume | 113 |
| Issue | 2 |
| Beginning Page | 279 |
| Ending Page | 291 |
| Digital Object Identifier (DOI) | 10.1007/s10482-019-01336-x |
| Page Count | 13 |
| Web of Science Category | Microbiology |
| Subject Category | Microbiology |
| Document Delivery Number | KJ4EF |
| Unique Article Identifier | WOS:000488944400001 |
