| Publication Type | J |
| Authors | Meng, Lin; Zhang, Lin; Guo, Qiang; Li, Shan-Shan; Mao, Pei-Chun; Tian, Xiao-Xia |
| Author Full Name | Meng, Lin; Zhang, Lin; Guo, Qiang; Li, Shan-Shan; Mao, Pei-Chun; Tian, Xiao-Xia |
| Title | Cloning and Transformation of EeHKT1;4 Gene from Elytrigia elongata |
| Source | PROTEIN AND PEPTIDE LETTERS |
| Language | English |
| Document Type | Article |
| Author Keywords | Clone; EeHKT1;4 gene; Elytrigia elongata (Host) Nevski; plant expression vector; transformation; transgenic tobacco |
| Keywords Plus | HKT TRANSPORTERS; SALT TOLERANCE; SODIUM EXCLUSION; CATION TRANSPORTER; KEY DETERMINANTS; SALINE SOILS; ORYZA-SATIVA; GRAIN-YIELD; DURUM-WHEAT; NA+ UPTAKE |
| Abstract | The EeHKT1;4 gene was firstly cloned from Elytrigia elongata by RT-PCR technique with 1977 bp full-length cDNA encoding 1722 bp open reading frame (ORF) and 573 amino acids. The PCR fragment of EeHKT1;4 gene was inserted into the binary vector pBI121 and got the resulted expression vector, which named pBI121-35S-EeHKT1;4-Nos. The vector was further transformed into the agrobacterium EHA105, and then EeHKT1;4 gene was transferred into tobacco by the Agrobaterium-mediated genetic transformation method. The results showed that the target gene was inserted into the genomes of tobacco and expressed. Therefore, the transgenic tobacco (T-0) plants overexpressing EeHKT1;4 gene were successfully obtained in this study. And EeHKT1;4 reduces Na+ concentration in the leaves of T-0 plants, thereby plays a central role in protecting plant leaves from salinity stress. |
| Author Address | [Meng, Lin; Zhang, Lin; Guo, Qiang; Li, Shan-Shan; Mao, Pei-Chun; Tian, Xiao-Xia] Beijing Acad Agr & Forestry Sci, Beijing Res & Dev Ctr Grass & Environm, Beijing 100097, Peoples R China |
| Reprint Address | Meng, L (reprint author), Beijing Acad Agr & Forestry Sci, Beijing Res & Dev Ctr Grass & Environm, Beijing 100097, Peoples R China. |
| E-mail Address | menglin9599@sina.com |
| Funding Agency and Grant Number | National Natural Science Foundation of China [31272489]; Scientific Innovation Ability Construction Project of Beijing Academy of Agriculture and Forestry Sciences (BAAFS) [KJCX20140103] |
| Funding Text | This work was supported by National Natural Science Foundation of China (Grant No. 31272489) and Scientific Innovation Ability Construction Project of Beijing Academy of Agriculture and Forestry Sciences (BAAFS) (Grant No. KJCX20140103). |
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| Cited Reference Count | 28 |
| Times Cited | 0 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 0 |
| Publisher | BENTHAM SCIENCE PUBL LTD |
| Publisher City | SHARJAH |
| Publisher Address | EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES |
| ISSN | 0929-8665 |
| 29-Character Source Abbreviation | PROTEIN PEPTIDE LETT |
| ISO Source Abbreviation | Protein Pept. Lett. |
| Year Published | 2016 |
| Volume | 23 |
| Issue | 5 |
| Beginning Page | 488 |
| Ending Page | 494 |
| Digital Object Identifier (DOI) | 10.2174/0929866523666160314152116 |
| Page Count | 7 |
| Subject Category | Biochemistry & Molecular Biology |
| Unique Article Identifier | WOS:000373716000010 |
